Category: ScienceEveryday (medical imaging is separate)

September 14, 2016 ScienceEveryday (medical imaging is separate) Post a Comment

Mutations and resistance

Mutations and resistance

This is a timely post by Buddhini Samarasinghe considering the FDA in the USA has finally issued a final rule on antibacterial soap.

Today (Sept. 2), the US Food and Drug Administration issued a final rule designed to protect the American public from these chemicals and their marketers: Moving forward, companies will no longer be allowed to market any antibacterial washes that contain one or more of 19 specific active ingredients. The new rule, initially proposed in 2013, gives companies a year to reformulate their products without those chemicals.

“Consumers may think antibacterial washes are more effective at preventing the spread of germs, but we have no scientific evidence that they are any better than plain soap and water,” said Dr. Janet Woodcock, director of the FDA’s Center for Drug Evaluation and Research, in an agency press release. “In fact, some data suggests that antibacterial ingredients may do more harm than good over the long-term.”

https://plus.google.com/u/0/+WardPlunet/posts/RSCtpixYhWm

h/t to Ward Plunet for the soap post

Originally shared by Buddhini Samarasinghe

The Evolution of Antibiotic Resistance

One of my favourite things about my job at the MRC is writing about all types of biomedical research, and not just cancer. This week I’ve been digging into the threat of antibiotic resistance, and it is truly sobering to read how bad a problem it is. Coincidentally I stumbled across this fantastic experiment carried out by a team of researchers at Harvard University. It illustrates how antibiotic resistance happens, and more importantly (and scarily!) how fast it happens. I love this experiment for the simplicity behind it, and how illuminating the results are.

✤ To study the evolution of antibiotic resistance, the researchers set up a giant petri dish. This rectangular dish was filled with agar that was dyed black (so that the bacteria could be seen easily) and topped off with soft agar (so that the bacteria could move easily).

✤ The plate was divided up into nine sections for antibiotic concentration. The outmost edges of the plate had no antibiotic, and then the dose was gradually increased until the centre of the plate had 3000 units of antibiotic.

✤ The researchers then put E. coli bacteria on the edge of the plate. These bacteria are able to move, and therefore when they used up all the nutrients in a local area, they spread through a mechanism called ‘chemotaxis’ – the bacteria are drawn towards the chemicals released by the nutrients in the nearby regions on the agar plate. But these nearby regions have antibiotics in them, so only bacteria that have evolved resistance can spread to these regions.

✤ It’s worth noting that the antibiotic starts out at a non-lethal dose, which means that a certain proportion of the bacteria will be able to survive it. Then, as the antibiotic gradually increases, it selects the bacteria that have mutations in their genes that allow them to survive despite ever-increasing concentrations of antibiotic.

✤ The beauty of this experiment is that it is possible to see this happening in the relatively short space of 11 days. What’s more, the researchers were able to sample the resistant bacteria and then sequence the genes to find out exactly how antibiotic resistance evolves.

✤ One of the most common mutations was in a gene known as dnaQ which codes for a protein that helps copy DNA when the cells divide. This protein has proof-reading abilities, but when it is mutated, the proof-reading ‘relaxes’ – resulting in a typo-ridden genome that has immense potential for accumulating more and more mutations very rapidly. In addition to dnaQ mutations, the bacteria also had mutations in genes involved in the folate biosynthesis pathway, which is the main target of the antibiotic used in this experiment.

✤ It’s also worth noting that mutations that increased antibiotic resistance came at a cost – these mutant bacterial strains were smaller due to reduced growth. But as soon as the mutants established themselves in the antibiotic-filled region, compensatory mutations kicked in and they were able to reach normal size.

✤ In the end, the bacteria at the centre of the plate were able to tolerate a dose of antibiotics that was 1000 times higher than that tolerated by the starting bacteria. It’s terrifying to realise how under the right conditions, bacteria can evolve so quickly. It’s also sobering to realise that antibiotics, a discovery that transformed modern medicine, may soon be obsolete thanks to the wide-spread misuse of antibiotics in agriculture and medical settings.

Full text research paper: http://science.sciencemag.org/content/353/6304/1147.full

More writing on antibiotic resistance: https://www.statnews.com/2016/09/12/superbug-antibiotic-resistance-history/ and http://phenomena.nationalgeographic.com/2015/11/21/mcr-gene-colistin/

https://www.youtube.com/watch?v=yybsSqcB7mE

August 23, 2016 ScienceEveryday (medical imaging is separate) Post a Comment

Really old dog, new tricks findings?

Really old dog, new tricks findings?

I’m looking forward to the findings from this excavation at the Lima Zoo in Peru. 1000-year-old dogs were buried with humans (126 humans and 128 dogs) and they were discovered beneath the zoo. There appears to be a variety of breeds. One interesting thing that I learned is that some pre-Columbian Peruvian cultures believed that dogs escorted humans to the afterlife.

#ScienceEveryday  

http://www.sciencemag.org/news/2016/08/why-are-these-1000-year-old-dogs-buried-under-lima-zoo

June 8, 2016 ScienceEveryday (medical imaging is separate) Post a Comment

Just say no to blots

Just say no to blots

I made a gel once or twice when I was in grad school. My advisor was too cheap. I hated it. When you pull the comb out that make the ‘starting wells’, you ruin half of the wells. Of course this was before PCR so I was making gels for gel electrophoresis.

Buddhini Samarasinghe knows what I’m talking about.

h/t mary Zeman 

Originally shared by Beth Osia

I blame the primers.

http://i.imgur.com/6aF6RqG.jpg

May 23, 2016 ScienceEveryday (medical imaging is separate) Post a Comment

FOIA tool for harassment

FOIA tool for harassment

I’ve posted before about Kevin Folta’s previous FOIA episode.

https://plus.google.com/u/0/+ChadHaney/posts/96vXnYoykFM

I guess when science isn’t on your side, you use tactics like senseless FOIA requests until the scientists give up.

Originally shared by David Wood

At what point does the FOIA stop being used for legitimate uses and starts being a tool for one egomaniacs paranoid obsession with herself. Good luck Kevin Folta

May 12, 2016 ScienceEveryday (medical imaging is separate) Post a Comment

A new study finds..

A new study finds..

A lot of you have probably already seen this episode of Last Week Tonight. You can probably guess that John Oliver’s message resonates with me.

Here are examples of busting media hype around science findings. I really enjoy busing the hype.

Dangerous Vehicle

https://plus.google.com/u/0/+ChadHaney/posts/7VjZitUZoMZ

Pulling a Fast One on Cancer via Buddhini Samarasinghe 

https://plus.google.com/u/0/+BuddhiniSamarasinghe/posts/bqbM8g9xwsv

Silver Lining in News of a Silver Bullet

https://plus.google.com/u/0/+ChadHaney/posts/Dps9EiJNtLA

Clean up on aisle 9

https://plus.google.com/u/0/+ChadHaney/posts/JdbtTvdB9vx

Still not much info and trouble brewing for Theranos

https://plus.google.com/u/0/+ChadHaney/posts/dywrVMavLBR

Overselling preclinical results

https://plus.google.com/u/0/+ChadHaney/posts/UWn7Q9hTuHm

Don’t hyperventilate

https://plus.google.com/u/0/+ChadHaney/posts/2t3JJFikwcs

Alarming science discovery…

https://plus.google.com/u/0/+ChadHaney/posts/LqAHEDPPgYt

http://www.youtube.com/watch?v=0Rnq1NpHdmw